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Identification and characterization of chitinase producing marine microorganism: Unleashing the potential of chitooligosaccharides for bioethanol synthesis.
P V, Atheena; K M, Rajesh; Raval, Keyur; Selvaraj, Subbalaxmi; Raval, Ritu.
Afiliação
  • P V A; Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.
  • K M R; Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India.
  • Raval K; Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, Mangalore 575025, Karnataka, India.
  • Selvaraj S; Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India. Electronic address: subbalaxmi.s@manipal.edu.
  • Raval R; Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India. Electronic address: ritu.raval@manipal.edu.
Int J Biol Macromol ; 265(Pt 1): 130846, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38492689
ABSTRACT
The dwindling supply of the petroleum product and its carbon footprint has initiated search for a sustainable fuel and alternate feed-stocks. One such underexplored feedstock is chitin, a waste derived from sea food processing. The limitation of insolubility and crystallinity inherent in chitin is addressed with the chitin hydrolysates. In the present study, a chitinases producing marine isolate was isolated from the sediments of Arabian Sea from a depth of 20 m. In order to increase the expression of the chitinases, sequential optimisation using one factor at a time and Taguchi experimental designs were employed which resulted in a yield of 13.46 U/mL which was 2.62 fold higher than the initial bioprocess condition values. In a two-step refinery protocol, Candida albicans was evolved towards chitooligosaccharides using chemically synthesized hydrolysates. In a fed -batch fermentation design the Candida yielded a 12.8 % conversion of these commercial chitin oligosaccharides into bioethanol in a run time of 48 h. This is the first report demonstrating the potential of Candida to utilise chitin oligosaccharides for the production of bioethanol.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Quitinases / Quitosana Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Quitinases / Quitosana Idioma: En Ano de publicação: 2024 Tipo de documento: Article