Dermal absorption of high molecular weight parent and alkylated polycyclic aromatic hydrocarbons from manufactured gas plant soils using in vitro assessment.

ABSTRACT

An enhanced in vitro human dermal bioavailability method was developed to measure the release of twenty parent and seven alkylated high molecular weight (HMW) polycyclic aromatic hydrocarbons (PAHs) from contaminated soils collected from five former manufactured Gas Plants (MGP) in England. GC-MS/MS was used to quantify HMW PAHs in soil, Strat-M artificial membrane representing skin, and synthetic receptor solution (RS) representing systemic circulation at 1-h, 10-h, and 24-h timesteps. Fluoranthene and pyrene exhibited the highest fluxes from soils to membrane (ranging from 9.5 - 281 ng/cm2/h) and soil to RS (absorption varies between both HMW parent and alkylated PAHs and individual PAHs. The utilisation of real-world contaminated soils allowed for a more realistic representation; this is important because current risk assessment guidance is baseed on results from experiments that used artificially spiked soils. This research shows that the the ranges of dermal fluxes are PAH dependent and impact the mass of absorbed from soil after dermal exposure and therefore the potential risk contaminated soil poses to human health.