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Triplex DNA-based aggregation-induced emission probe: A new platform for hybridization chain reaction-based fluorescence sensing assay.
Li, Yubin; Li, Ciling; Zhang, Chuangqiang; Zhao, Liting; Huang, Yaoyun.
Afiliação
  • Li Y; Faculty of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China; Research Center for Coastal Environmental Protection and Ecological Resilience, Guangdong Ocean University, Zhanjiang, 524088, China. Electronic address: liyb@gdou.edu.cn.
  • Li C; Faculty of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China.
  • Zhang C; Faculty of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China.
  • Zhao L; Faculty of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China.
  • Huang Y; Faculty of Chemistry & Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China.
Anal Chim Acta ; 1299: 342406, 2024 Apr 22.
Article em En | MEDLINE | ID: mdl-38499412
ABSTRACT
The hybridization chain reaction (HCR), as one of the nucleic acid amplification technologies, is combined with fluorescence signal output with excellent sensitivity, simplicity, and stability. However, current HCR-based fluorescence sensing methods still have some defects such as the blocking effect of the HCR combination with fluorophores and the aggregation-caused quenching (ACQ) phenomenon of traditional fluorophores. Herein, a triplex DNA-based aggregation-induced emission probe (AIE-P) was designed as the fluorescent signal transduction, which is able to provide a new platform for HCR-based sensing assay. The AIE-P was synthesized by attaching the AIE fluorophores to terminus of the oligonucleotide through amido bond, and captured the products of HCR to form triplex DNA. In this case, the AIE fluorophores were located in close proximity to generate fluorescence. This assay provided turn-on fluorescence efficiency with a high signal-to-noise ratio and excellent amplification capability to solve the shortcoming of HCR-based fluorescence sensing methods. It enabled sensitive detection of Vibrio parahaemolyticus in the range of 102-106 CFU mL-1, and with a low limit of detection down to 39 CFU mL-1. In addition, this assay expressed good specificity and practicability. The triplex DNA-based AIE probe forms a universal molecular tool for developing HCR-based fluorescence sensing methods.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Técnicas Biossensoriais Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Técnicas Biossensoriais Idioma: En Ano de publicação: 2024 Tipo de documento: Article