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Myocardin reverses insulin resistance and ameliorates cardiomyopathy by increasing IRS-1 expression in a murine model of lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase V0d1 subunit.
Yuan, Wenlin; Lin, Hui; Sun, Yuan; Liu, Lihuan; Yan, Meijuan; Song, Yujuan; Zhang, Xiaofan; Lu, Xiangling; Xu, Yipei; He, Qiyue; Ouyang, Kunfu; Zhang, Chenglin; Pan, Yong; Huang, Yu; Li, Ying; Lu, Xifeng; Liu, Jie.
Afiliação
  • Yuan W; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Lin H; Clinical Research Center, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.
  • Sun Y; Division of Pharmacology and Vascular Medicine, Department of Internal Medicine, Erasmus Medical Center, Rotterdam, the Netherlands.
  • Liu L; Clinical Research Center, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.
  • Yan M; Department of Pharmacology, College of Pharmacy, Shenzhen Technology University, Shenzhen, China.
  • Song Y; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Zhang X; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Lu X; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Xu Y; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • He Q; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Ouyang K; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Zhang C; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Pan Y; Department of Cardiovascular Surgery, Peking University Shenzhen Hospital, Shenzhen, China.
  • Huang Y; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Li Y; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
  • Lu X; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
  • Liu J; Department of pathophysiology, Shenzhen University Medical School, Shenzhen University, Shenzhen, China.
Theranostics ; 14(5): 2246-2264, 2024.
Article em En | MEDLINE | ID: mdl-38505620
ABSTRACT

Aim:

Adipose tissue (AT) dysfunction that occurs in both obesity and lipodystrophy is associated with the development of cardiomyopathy. However, it is unclear how dysfunctional AT induces cardiomyopathy due to limited animal models available. We have identified vacuolar H+-ATPase subunit Vod1, encoded by Atp6v0d1, as a master regulator of adipogenesis, and adipose-specific deletion of Atp6v0d1 (Atp6v0d1AKO) in mice caused generalized lipodystrophy and spontaneous cardiomyopathy. Using this unique animal model, we explore the mechanism(s) underlying lipodystrophy-related cardiomyopathy. Methods and

Results:

Atp6v0d1AKO mice developed cardiac hypertrophy at 12 weeks, and progressed to heart failure at 28 weeks. The Atp6v0d1AKO mouse hearts exhibited excessive lipid accumulation and altered lipid and glucose metabolism, which are typical for obesity- and diabetes-related cardiomyopathy. The Atp6v0d1AKO mice developed cardiac insulin resistance evidenced by decreased IRS-1/2 expression in hearts. Meanwhile, the expression of forkhead box O1 (FoxO1), a transcription factor which plays critical roles in regulating cardiac lipid and glucose metabolism, was increased. RNA-seq data and molecular biological assays demonstrated reduced expression of myocardin, a transcription coactivator, in Atp6v0d1AKO mouse hearts. RNA interference (RNAi), luciferase reporter and ChIP-qPCR assays revealed the critical role of myocardin in regulating IRS-1 transcription through the CArG-like element in IRS-1 promoter. Reducing IRS-1 expression with RNAi increased FoxO1 expression, while increasing IRS-1 expression reversed myocardin downregulation-induced FoxO1 upregulation in cardiomyocytes. In vivo, restoring myocardin expression specifically in Atp6v0d1AKO cardiomyocytes increased IRS-1, but decreased FoxO1 expression. As a result, the abnormal expressions of metabolic genes in Atp6v0d1AKO hearts were reversed, and cardiac dysfunctions were ameliorated. Myocardin expression was also reduced in high fat diet-induced diabetic cardiomyopathy and palmitic acid-treated cardiomyocytes. Moreover, increasing systemic insulin resistance with rosiglitazone restored cardiac myocardin expression and improved cardiac functions in Atp6v0d1AKO mice.

Conclusion:

Atp6v0d1AKO mice are a novel animal model for studying lipodystrophy- or metabolic dysfunction-related cardiomyopathy. Moreover, myocardin serves as a key regulator of cardiac insulin sensitivity and metabolic homeostasis, highlighting myocardin as a potential therapeutic target for treating lipodystrophy- and diabetes-related cardiomyopathy.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Proteínas Nucleares / Transativadores / ATPases Vacuolares Próton-Translocadoras / Cardiomiopatias Diabéticas / Insuficiência Cardíaca / Lipodistrofia Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Resistência à Insulina / Proteínas Nucleares / Transativadores / ATPases Vacuolares Próton-Translocadoras / Cardiomiopatias Diabéticas / Insuficiência Cardíaca / Lipodistrofia Idioma: En Ano de publicação: 2024 Tipo de documento: Article