Identification of the novel fosfomycin resistance gene fosSC in Staphylococcus capitis.
Int J Antimicrob Agents
; 63(6): 107162, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38561093
ABSTRACT
OBJECTIVES:
Fosfomycin has regained attention for treating infections caused by methicillin-resistant Staphylococcus aureus and multidrug-resistant coagulase-negative staphylococci. In this research, our objective was to investigate the mechanisms underlying fosfomycin resistance in Staphylococcus capitis.METHODS:
The minimum inhibitory concentrations (MICs) of fosfomycin were assessed in 109 clinical S. capitis isolates by the agar dilution method. By cloning the fos-like genes into the shuttle vector, pTSSCm-Pcap, and observing the change in fosfomycin MICs, the gene function was verified. Core genome multilocus sequence typing and comparative genomics analysis were conducted to determine the population characteristics of S. capitis isolates and analyse the genetic environment of the fos-like genes.RESULTS:
We identified a novel fosfomycin resistance gene, fosSC, on the chromosome in 58 out of 109 (53.2%) S. capitis isolates. The deduced products of the fosSC genes shared 67.15-67.88% amino acid sequence identity with FosB. The RN-pT-fosSC transformants carrying fosSC showed a 512-fold increase in the fosfomycin MICs. The fosSC gene was embedded in a conserved genetic context, but IS431mec was located to the left of the fosSC gene in cluster L due to the insertion of staphylococcal cassette chromosome mec.CONCLUSIONS:
The chromosomal fosSC genes in some lineages of S. capitis explained their high-level fosfomycin resistance. Ongoing surveillance is crucial for monitoring the potential threat of horizontal transfer, which could be facilitated by the presence of mobile genetic elements surrounding the fosSC gene.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Infecções Estafilocócicas
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Testes de Sensibilidade Microbiana
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Farmacorresistência Bacteriana
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Staphylococcus capitis
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Fosfomicina
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Antibacterianos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article