Insertion of foreign genes into the simian varicella virus genome by Tn7-mediated site-specific transposition.
J Virol Methods
; 327: 114936, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38583808
ABSTRACT
A Tn7-transposition approach was utilized for site-specific insertion of foreign genes into the genome of simian varicella virus (SVV), the causative agent of simian varicella in nonhuman primates. The severe acute respiratory syndrome coronavirus (SARS-CoV-2) nucleocapsid (N) gene and receptor binding domain (RBD) of the spike gene were inserted into the ORF 14 region of the SVV genome cloned into a bacterial artificial chromosome and then transfected into Vero cells to generate the infectious recombinant SVV (rSVV). The rSVV replicated efficiently in infected Vero cells and expressed the N and RBD antigens as indicated by immunoblot and immunofluorescence assays. Tn7-mediated transposition provides a rapid and efficient method for constructing rSVVs which may be evaluated as live-attenuated vaccines.
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MEDLINE
Assunto principal:
Genoma Viral
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article