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Proteomic analyses on chicken breast meat with white striping myopathy.
Kong, Byungwhi; Owens, Casey; Bottje, Walter; Shakeri, Majid; Choi, Janghan; Zhuang, Hong; Bowker, Brian.
Afiliação
  • Kong B; USDA, Agricultural Research Service, U.S. National Poultry Research Center, Quality & Safety Assessment Research Unit, Athens, GA, USA. Electronic address: byungwhi.kong@usda.gov.
  • Owens C; Department of Poultry Science, Division of Agriculture, University of Arkansas System, Fayetteville, AR, USA.
  • Bottje W; Department of Poultry Science, Division of Agriculture, University of Arkansas System, Fayetteville, AR, USA.
  • Shakeri M; USDA, Agricultural Research Service, U.S. National Poultry Research Center, Quality & Safety Assessment Research Unit, Athens, GA, USA.
  • Choi J; USDA, Agricultural Research Service, U.S. National Poultry Research Center, Quality & Safety Assessment Research Unit, Athens, GA, USA.
  • Zhuang H; USDA, Agricultural Research Service, U.S. National Poultry Research Center, Quality & Safety Assessment Research Unit, Athens, GA, USA.
  • Bowker B; USDA, Agricultural Research Service, U.S. National Poultry Research Center, Quality & Safety Assessment Research Unit, Athens, GA, USA.
Poult Sci ; 103(6): 103682, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38593545
ABSTRACT
White striping (WS) is an emerging myopathy that results in significant economic losses as high as $1 billion (combined with losses derived from other breast myopathies including woody breast and spaghetti meat) to the global poultry industry. White striping is detected as the occurrence of white lines on raw poultry meat. The exact etiologies for WS are still unclear. Proteomic analyses of co-expressed WS and woody breast phenotypes previously demonstrated dysfunctions in carbohydrate metabolism, protein synthesis, and calcium buffering capabilities in muscle cells. In this study, we conducted shotgun proteomics on chicken breast fillets exhibiting only WS that were collected at approximately 6 h postmortem. After determining WS severity, protein extractions were conducted from severe WS meat with no woody breast (WB) condition (n = 5) and normal non-affected (no WS) control meat (n = 5). Shotgun proteomics was conducted by Orbitrap Lumos, tandem mass tag (TMT) analysis. As results, 148 differentially abundant proteins (|fold change|>1.4; p-value < 0.05) were identified in the WS meats compared with controls. The significant canonical pathways included BAG2 signaling pathway, glycogen degradation II, isoleucine degradation I, aldosterone signaling in epithelial cells, and valine degradation I. The potential upstream regulators include LIPE, UCP1, ATP5IF1, and DMD. The results of this study provide additional insights into the cellular mechanisms on the WS myopathy and meat quality.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Músculos Peitorais / Doenças das Aves Domésticas / Galinhas / Proteínas Aviárias / Proteômica / Carne / Doenças Musculares Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Músculos Peitorais / Doenças das Aves Domésticas / Galinhas / Proteínas Aviárias / Proteômica / Carne / Doenças Musculares Idioma: En Ano de publicação: 2024 Tipo de documento: Article