The Schizosaccharomyces pombe DEAD-box protein Mss116 is required for mitoribosome assembly and mitochondrial translation.
Mitochondrion
; 76: 101881, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38604460
ABSTRACT
DEAD-box helicases are important players in mitochondrial gene expression, which is necessary for mitochondrial respiration. In this study, we characterized Schizosaccharomyces pombe Mss116 (spMss116), a member of the family of DEAD-box RNA helicases. Deletion of spmss116 in a mitochondrial intron-containing background significantly reduced the levels of mitochondrial DNA (mtDNA)-encoded cox1 and cob1 mRNAs and impaired mitochondrial translation, leading to a severe respiratory defect and a loss of cell viability during stationary phase. Deletion of mitochondrial introns restored the levels of cox1 and cob1 mRNAs to wide-type (WT) levels but could not restore mitochondrial translation and respiration in Δspmss116 cells. Furthermore, deletion of spmss116 in both mitochondrial intron-containing and intronless backgrounds impaired mitoribosome assembly and destabilization of mitoribosomal proteins. Our findings suggest that defective mitochondrial translation caused by deletion of spmss116 is most likely due to impaired mitoribosome assembly.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Biossíntese de Proteínas
/
Proteínas de Schizosaccharomyces pombe
/
RNA Helicases DEAD-box
/
Ribossomos Mitocondriais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article