A Robust In Vitro Culture Model and Generation of Memory Monocytes.
Methods Mol Biol
; 2782: 81-88, 2024.
Article
em En
| MEDLINE
| ID: mdl-38622393
ABSTRACT
Innate monocytes can be trained or reprogrammed to adopt distinct memory states, such as low-grade inflammation and immune exhaustion, bearing fundamental relevance to the pathogenesis of both acute diseases such as sepsis as well as chronic diseases such as atherosclerosis. Therefore, it is critically important to develop a regimen for generating memory monocytes in vitro in order to better define key monocyte memory states with diverse potentials for proliferation, differentiation, and activation, as well as underlying mechanisms. Here, we describe an efficient in vitro system to propagate a large number of highly purified murine memory monocytes through sustaining bone marrow-derived monocytes with macrophage colony-stimulating factor (M-CSF, 10 ng/mL)-containing medium, together with other polarization agents such as lipopolysaccharide (LPS) for a 5-day period. This method can yield high-purity monocytes, capable of exhibiting dynamic memory behaviors upon training with various polarizing agents.
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MEDLINE
Assunto principal:
Monócitos
/
Fator Estimulador de Colônias de Macrófagos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article