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C-type lectin 4 of Toxocara canis activates NF-ĸB and MAPK pathways by modulating NOD1/2 and RIP2 in murine macrophages in vitro.
Wu, Tian-Le; Wang, Bing-Nan; Yang, Ai-Jia; Wang, Lei; You, Yi-Ning; Zhou, Rong-Qiong.
Afiliação
  • Wu TL; College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  • Wang BN; College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  • Yang AJ; College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  • Wang L; College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  • You YN; College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.
  • Zhou RQ; College of Veterinary Medicine, Southwest University, Chongqing, 402460, China. rongqiongzhou@126.com.
Parasitol Res ; 123(4): 189, 2024 Apr 19.
Article em En | MEDLINE | ID: mdl-38639821
ABSTRACT
Toxocara canis is a parasitic zoonose that is distributed worldwide and is one of the two pathogens causing toxocariasis. After infection, it causes serious public health and safety problems, which pose significant veterinary and medical challenges. To better understand the regulatory effects of T. canis infection on the host immune cells, murine macrophages (RAW264.7) were incubated with recombinant T. canis C-type lectin 4 (rTc-CTL-4) protein in vitro. The quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to analyze the nucleotide-binding oligomerization domain-containing protein 1/2 (NOD1/2), receptor-interacting protein 2 (RIP2), nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB), and mitogen-activated protein kinase (MAPK) on mRNA level and protein expression level in macrophages. Our results indicated that 10 µg/mL rTc-CTL-4 protein could modulate the expression of NOD1, NOD2, and RIP2 at both the transcriptional and translational levels. The protein translation levels of NF-κB, P-p65, p38, and P-p38 in macrophages were also modulated by rTc-CTL-4 protein. Macrophages were co-incubated with rTc-CTL-4 protein after siRNA silencing of NOD1, NOD2, and RIP2. The expression levels of NF-κB, P-p65, p38, and P-p38 were significantly changed compared with the negative control groups (Neg. Ctrl.). Taken together, rTc-CTL-4 protein seemed to act on NOD1/2-RIP2-NF-κB and MAPK signaling pathways in macrophages and might activate MAPK and NF-κB signaling pathways by regulating NOD1, NOD2, and RIP2. The insights from the above studies could contribute to our understanding of immune recognition and regulatory mechanisms of T. canis infection in the host animals.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: NF-kappa B / Toxocara canis Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: NF-kappa B / Toxocara canis Idioma: En Ano de publicação: 2024 Tipo de documento: Article