Measurements of Oxygen Evolution in Photosynthesis.
Methods Mol Biol
; 2790: 133-148, 2024.
Article
em En
| MEDLINE
| ID: mdl-38649570
ABSTRACT
This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
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Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Fotossíntese
/
Espectrometria de Massas
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Complexo de Proteína do Fotossistema II
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article