Loss of AMPK activity induces organelle dysfunction and oxidative stress during oocyte aging.
Biol Direct
; 19(1): 29, 2024 Apr 23.
Article
em En
| MEDLINE
| ID: mdl-38654312
ABSTRACT
BACKGROUND:
Oocyte quality is critical for the mammalian reproduction due to its necessity on fertilization and early development. During aging, the declined oocytes showing with organelle dysfunction and oxidative stress lead to infertility. AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase which is important for energy homeostasis for metabolism. Little is known about the potential relationship between AMPK with oocyte aging.RESULTS:
In present study we reported that AMPK was related with low quality of oocytes under post ovulatory aging and the potential mechanism. We showed the altered AMPK level during aging and inhibition of AMPK activity induced mouse oocyte maturation defect. Further analysis indicated that similar with its upstream regulator PKD1, AMPK could reduce ROS level to avoid oxidative stress in oocytes, and this might be due to its regulation on mitochondria function, since loss of AMPK activity induced abnormal distribution, reduced ATP production and mtDNA copy number of mitochondria. Besides, we also found that the ER and Golgi apparatus distribution was aberrant after AMPK inhibition, and enhanced lysosome function was also observed.CONCLUSIONS:
Taken together, these data indicated that AMPK is important for the organelle function to reduce oxidative stress during oocyte meiotic maturation.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Estresse Oxidativo
/
Proteínas Quinases Ativadas por AMP
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article