IFT cargo and motors associate sequentially with IFT trains to enter cilia of C. elegans.
Nat Commun
; 15(1): 3456, 2024 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-38658528
ABSTRACT
Intraflagellar transport (IFT) orchestrates entry of proteins into primary cilia. At the ciliary base, assembled IFT trains, driven by kinesin-2 motors, can transport cargo proteins into the cilium, across the crowded transition zone. How trains assemble at the base and how proteins associate with them is far from understood. Here, we use single-molecule imaging in the cilia of C. elegans chemosensory neurons to directly visualize the entry of kinesin-2 motors, kinesin-II and OSM-3, as well as anterograde cargo proteins, IFT dynein and tubulin. Single-particle tracking shows that IFT components associate with trains sequentially, both in time and space. Super-resolution maps of IFT components in wild-type and mutant worms reveal ciliary ultrastructure and show that kinesin-II is essential for axonemal organization. Finally, imaging cilia lacking kinesin-II and/or transition zone function uncovers the interplay of kinesin-II and OSM-3 in driving efficient transport of IFT trains across the transition zone.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cílios
/
Cinesinas
/
Caenorhabditis elegans
/
Proteínas de Caenorhabditis elegans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article