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A Water-Soluble Wavy Coordination Polymer of Cu(II) as a Turn-On Luminescent Probe for Histidine and Histidine-Rich Proteins/Peptides.
Ghosh, Riya; Nair, Ratish R; Ghosh, Shibaji; Debnath, Snehasish; Chatterjee, Pabitra B.
Afiliação
  • Ghosh R; Analytical & Environmental Science Division and Centralized Instrument Facility, CSIR-CSMCRI, G. B. Marg, Bhavnagar 364002, India.
  • Nair RR; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Ghosh S; Analytical & Environmental Science Division and Centralized Instrument Facility, CSIR-CSMCRI, G. B. Marg, Bhavnagar 364002, India.
  • Debnath S; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Chatterjee PB; Analytical & Environmental Science Division and Centralized Instrument Facility, CSIR-CSMCRI, G. B. Marg, Bhavnagar 364002, India.
Inorg Chem ; 63(18): 8320-8328, 2024 May 06.
Article em En | MEDLINE | ID: mdl-38660721
ABSTRACT
Histidine plays an essential role in most biological systems. Changes in the homeostasis of histidine and histidine-rich proteins are connected to several diseases. Herein, we report a water-soluble Cu(II) coordination polymer, labeled CuCP, for the fluorimetric detection of histidine and histidine-rich proteins and peptides. Single-crystal structure determination of CuCP revealed a two-dimensional wavy network structure in which a carboxylate group connects the individual Cu(II) dimer unit in a syn-anti conformation. The weakly luminescent and water-soluble CuCP shows turn-on blue emission in the presence of histidine and histidine-rich peptides and proteins. The polymer can also stain histidine-rich proteins via gel electrophoresis. The limits of quantifications for histidine, glycine-histidine, serine-histidine, human serum albumin (HSA), bovine serum albumin, pepsin, trypsin, and lysozyme were found to be 300, 160, 600, 300, 600, 800, 120, and 290 nM, respectively. Utilizing the fluorescence turn-on property of CuCP, we measured HSA quantitatively in the urine samples. We also validated the present urinary HSA measurement assay with existing analytical techniques. Job's plot, 1H NMR, high-resolution mass spectrometry (HRMS), electron paramagnetic resonance (EPR), fluorescence, and UV-vis studies confirmed the ligand displacement from CuCP in the presence of histidine.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos / Água / Proteínas / Cobre / Histidina Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos / Água / Proteínas / Cobre / Histidina Idioma: En Ano de publicação: 2024 Tipo de documento: Article