Sandwich-like voltametric immunosensing of interleukin-8 based on ß-cyclodextrin/carbon nanotubes and methylthionine chloride@UIO-66 framework.
Anal Sci
; 40(7): 1357-1363, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38662337
ABSTRACT
The level of interleukin-8 (IL-8) in the body is an effective factor for the early diagnosis of acute tubular necrosis and oral tumor. In this work, a novel sandwich-like voltametric immunosensor (SVS) of IL-8 was constructed by preparing ß-cyclodextrin/carbon nanotube (CD/CNT) to immobilize primary antibody (PAb) of IL-8 and UIO-66-NH2 MOFs structure to immobilize second antibody (SAb) and methylene blue (Mb) probe. In this designed SVS, the prepared CD/CNT nanohybrid with large surface area and conductivity can immobilize PAb via simple host-guest recognition, and UIO-66-NH2 provided an ideal platform to accommodate SAb and a large number of Mb molecules as signal-amplifier. In the existence of target IL-8, the current peak of Mb from the SVS assay increases with the increasement of IL-8 level. Through optimizing and adjusting various factors, a wide linearity (0.001-2.5 ng mL-1) and low analytical limit (0.2 pg mL-1) of IL-8 were realized, so it's expected the developed SVS strategy has significant applications for the detection of IL-8.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Interleucina-8
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Nanotubos de Carbono
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Beta-Ciclodextrinas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article