Spatiotemporal expression of AP-2/myosin â
¥ in mouse cochlear IHCs and correlation with auditory function.
Acta Otolaryngol
; 144(3): 198-206, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38662892
ABSTRACT
BACKGROUND:
Recycling of synaptic vesicles plays an important role in vesicle pool replenishment, neurotransmitter release and synaptic plasticity. Clathrin-mediated endocytosis (CME) is considered to be the main mechanism for synaptic vesicle replenishment. AP-2 (adaptor-related protein complex 2) and myosin â ¥ are known as key proteins that regulate the structure and dynamics of CME.OBJECTIVE:
This study aims to reveal the spatiotemporal expression of AP-2/myosin â ¥ in inner hair cells (IHCs) of the mouse cochlea and its correlation with auditory function. MATERIAL ANDMETHODS:
Immunofluorescence was used to detect the localization and expression of AP-2 and myosin â ¥ in cochlear hair cells (HCs) of CBA/CaJ mice of various ages. qRT-PCR was used to verify the differential expression of AP-2 and myosin â ¥ mRNA in the mouse cochlea, and ABR tests were administered to mice of various ages. A preliminary analysis of the correlation between AP-2/myosin â ¥ levels and auditory function was conducted.RESULTS:
AP-2 was located in the cytoplasmic region of IHCs and was mainly expressed in the basal region of IHCs and the area near ribbon synapses, while myosin â ¥ was expressed in the cytoplasmic region of IHCs and OHCs. Furthermore, AP-2 and myosin â ¥ were not significant detected in the cochleae of P7 mice; the expression level reached a peak at P35 and then decreased significantly with age. The expression patterns and expression levels of AP-2 and myosin â ¥ in the cochleae of the mice were consistent with the development of the auditory system. CONCLUSIONS ANDSIGNIFICANCE:
AP-2 and myosin â ¥ protein expression may differ in mice of different ages, and this variation probably leads to a difference in the efficiency in CME; it may also cause a defect in IHC function.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Células Ciliadas Auditivas Internas
/
Camundongos Endogâmicos CBA
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article