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Alleviating effect of vagus nerve cutting in Salmonella-induced gut infections and anxiety-like behavior via enhancing microbiota-derived GABA.
Zou, Qianhui; Han, Shiyao; Liang, Jiarui; Yan, Guiming; Wang, Qianxu; Wang, Yajie; Zhang, Zilong; Hu, Jun; Li, Jufang; Yuan, Tian; Liu, Zhigang.
Afiliação
  • Zou Q; Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
  • Han S; Molecular Biology Laboratory of Stem Cells and Anti-infection Medicine, College of Veterinary medicine, Northwest A&F University, Yangling, Shaanxi, China.
  • Liang J; Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
  • Yan G; Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
  • Wang Q; Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
  • Wang Y; Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
  • Zhang Z; College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, China.
  • Hu J; Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.
  • Li J; Heilongjiang Feihe Dairy Co., Ltd., Beijing, China.
  • Yuan T; Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong, China; Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, China.
  • Liu Z; Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China; Northwest A&F University Shenzhen Research Institute, Shenzhen, Guangdong, China; Shaanxi Precision Nutrition and Health Research Institute,
Brain Behav Immun ; 119: 607-620, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38663772
ABSTRACT
The vagus nerve, a pivotal link within the gut-brain axis, plays a critical role in maintaining homeostasis and mediating communication between the gastrointestinal tract and the brain. It has been reported that gastrointestinal infection by Salmonella typhimurium (S. typhimurium) triggers gut inflammation and manifests as anxiety-like behaviors, yet the mechanistic involvement of the vagus nerve remains to be elucidated. In this study, we demonstrated that unilateral cervical vagotomy markedly attenuated anxiety-like behaviors induced by S. typhimurium SL1344 infection in C57BL/6 mice, as evidenced by the open field test and marble burying experiment. Furthermore, vagotomy significantly diminished neuronal activation within the nucleus of the solitary tract and amygdala, alongside mitigating aberrant glial cell activation in the hippocampus and amygdala. Additionally, vagotomy notably decreases serum endotoxin levels, counters the increase in splenic Salmonella concentration, and modulates the expression of inflammatory cytokines-including IL-6, IL-1ß, and TNF-α-in both the gastrointestinal tract and brain, with a concurrent reduction in IL-22 and CXCL1 expression. This intervention also fostered the enrichment of beneficial gut microbiota, including Alistipes and Lactobacillus species, and augmented the production of gamma-aminobutyric acid (GABA) in the gut. Administration of GABA replicated the vagotomy's beneficial effects on reducing gut inflammation and anxiety-like behavior in infected mice. However, blockade of GABA receptors with picrotoxin abrogated the vagotomy's protective effects against gut inflammation, without influencing its impact on anxiety-like behaviors. Collectively, these findings suggest that vagotomy exerts a protective effect against infection by promoting GABA synthesis in the colon and alleviating anxiety-like behavior. This study underscores the critical role of the vagus nerve in relaying signals of gut infection to the brain and posits that targeting the gut-brain axis may offer a novel and efficacious approach to preventing gastrointestinal infections and associated behavioral abnormalities.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ansiedade / Vagotomia / Nervo Vago / Microbioma Gastrointestinal / Ácido gama-Aminobutírico / Camundongos Endogâmicos C57BL Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ansiedade / Vagotomia / Nervo Vago / Microbioma Gastrointestinal / Ácido gama-Aminobutírico / Camundongos Endogâmicos C57BL Idioma: En Ano de publicação: 2024 Tipo de documento: Article