Reduced ossification caused by 3D simulated microgravity exposure is short-term in larval zebrafish.
Life Sci Space Res (Amst)
; 41: 127-135, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38670639
ABSTRACT
Understanding how skeletal tissues respond to microgravity is ever more important with the increased interest in human space travel. Here, we exposed larval Danio rerio at 3.5 dpf to simulated microgravity (SMG) using a 3D mode of rotation in a ground-based experiment and then studied different cellular, molecular, and morphological bone responses both immediately after exposure and one week later. Our results indicate an overall decrease in ossification in several developing skeletal elements immediately after SMG exposure with the exception of the otoliths, however ossification returns to normal levels seven days after exposure. Coincident with the reduction in overall ossification tnfsf11 (RANKL) expression is highly elevated after 24 h of SMG exposure and also returns to normal levels seven days after exposure. We also show that genes associated with osteoblasts are unaffected immediately after SMG exposure. Thus, the observed reduction in ossification is primarily the result of a high level of bone resorption. This study sheds insight into the nuances of how osteoblasts and osteoclasts in the skeleton of a vertebrate organism respond to an external environmental disturbance, in this case simulated microgravity.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteogênese
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Peixe-Zebra
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Simulação de Ausência de Peso
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Larva
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article