Chip-based automated equipment for dual-mode point-of-care testing foodborne pathogens.
Biosens Bioelectron
; 257: 116338, 2024 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-38677017
ABSTRACT
Foodborne pathogens have a substantial bearing on food safety and environmental health. The development of automated, portable and compact devices is essential for the on-site and rapid point-of-care testing (POCT) of bacteria. Here, this work developed a micro-automated microfluidic device for detecting bacteria, such as Escherichia coli (E. coli) O157H7, using a seashell-like microfluidic chip (SMC) as an analysis and mixing platform. The automated device integrates a colorimetric/fluorescent system for the metabolism of copper (Cu2+) by E. coli affecting o-phenylenediamine (OPD) for concentration analysis. A smartphone was used to read the RGB data of the chip reaction reservoir to detect colorimetric and fluorescence patterns in the concentration range of 102-106 CFU mL-1. The automated device overcomes the low efficiency and tedious steps of traditional detection and enables high-precision automated detection that can be applied to POCT in the field, providing an ideal solution for broadening the application of E. coli detection.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Colorimetria
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Escherichia coli O157
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Cobre
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Desenho de Equipamento
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Dispositivos Lab-On-A-Chip
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Testes Imediatos
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Microbiologia de Alimentos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article