Determination of optimum levels of binding antibody units (BAU) of new quantitative chemiluminescent immuno-assay (CLIA) in COVID-19 vaccinated volunteer blood donors.
Transfus Apher Sci
; 63(3): 103937, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38678985
ABSTRACT
BACKGROUND:
For assessment of COVID-19 vaccine efficacy, neutralization activity of anti-SARS-CoV-2 antibody is measured. This study was undertaken to determine optimum levels of binding antibody units (BAU/ml) in new quantitative chemiluminescent assay (CLIA) that corresponded to neutralizing potential (30% inhibition) of sVNT assay.METHODS:
Ninety-one blood samples were analyzed by CLIA and sVNT assays. Test samples (n = 75) were collected from blood donors post-2nd vaccination dose, while control samples (n = 16) were archived pre-COVID donor samples. Correlation between CLIA and sVNT was calculated and receiver operating characteristic (ROC) curve was drawn and analyzed.RESULTS:
Results indicated excellent correlation between 57.5 BAU/ml on CLIA and 30%inhibition on sVNT assay. ROC curve analysis revealed that the area under the curve (AUC) was 0.971.DISCUSSION:
The present study determined that 57.5 BAU/ml on CLIA corresponded to 30% inhibition on sVNT assay. Periodic quantitative analysis.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Doadores de Sangue
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Vacinas contra COVID-19
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SARS-CoV-2
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COVID-19
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Medições Luminescentes
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Anticorpos Antivirais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article