KpsS1 Mediates the Glycosylation of Pseudaminic Acid in Acinetobacter Baumannii.
Chemistry
; 30(41): e202400703, 2024 Jul 19.
Article
em En
| MEDLINE
| ID: mdl-38682414
ABSTRACT
Pseudaminic acid (Pse) is found in the polysaccharide structures of the cell surface of various Gram-negative pathogenic bacteria including Acinetobacter baumannii and considered as an important component of cell surface glycans including oligosaccharides and glycoproteins. However, the glycosyltransferase that is responsible for the Pse glycosylation in A. baumannii remains unknown yet. In this study, through comparative genomics analysis of Pse-positive and negative A. baumannii clinical isolates, we identified a potential glycosyltransferase, KpsS1, located right downstream of the Pse biosynthesis genetic locus. Deletion of this gene in an Pse-positive A. baumannii strain, Ab8, impaired the glycosylation of Pse to the surface CPS and proteins, while the gene knockout strain, Ab8ΔkpsS1, could still produce Pse with 2.86 folds higher amount than that of Ab8. Furthermore, impairment of Pse glycosylation affected the morphology and virulence potential of A. baumannii, suggesting the important role of this protein. This study will provide insights into the further understanding of Pse in bacterial physiology and pathogenesis.
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Base de dados:
MEDLINE
Assunto principal:
Glicosiltransferases
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Acinetobacter baumannii
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article