Protocol for high-throughput DNA methylation profiling in rat tissues using automated reduced representation bisulfite sequencing.
STAR Protoc
; 5(2): 103007, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38691461
ABSTRACT
Although reduced representation bisulfite sequencing (RRBS) measures DNA methylation (DNAme) across CpG-rich genomic regions with high sensitivity, the assay can be time-consuming and prone to batch effects. Here, we present a high-throughput, automated RRBS protocol starting with DNA extraction from frozen rat tissues. We describe steps for RRBS library preparation, library quality control, and sequencing. We also detail an optimized pipeline for sequencing data processing. This protocol has been applied successfully to DNAme profiling across multiple rat tissues. For complete details on the use and execution of this protocol, please refer to Nair et al.1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfitos
/
Metilação de DNA
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article