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A specific natural killer cells phenotypic signature associated to long term elite control of HIV infection.
Rallón, Norma; Jiménez-Carretero, Daniel; Restrepo, Clara; Ligos, José M; Valentín-Quiroga, Jaime; Mahillo, Ignacio; Cabello, Alfonso; López-Collazo, Eduardo; Sánchez-Cabo, Fátima; Górgolas, Miguel; Estrada, Vicente; Benito, José M.
Afiliação
  • Rallón N; HIV and Viral Hepatitis Research Laboratory, Instituto de Investigación Sanitaria Fundación Jiménez Díaz, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain.
  • Jiménez-Carretero D; Hospital Universitario Rey Juan Carlos, Móstoles, Spain.
  • Restrepo C; Unidad de Bioinformática, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
  • Ligos JM; HIV and Viral Hepatitis Research Laboratory, Instituto de Investigación Sanitaria Fundación Jiménez Díaz, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain.
  • Valentín-Quiroga J; Hospital Universitario Rey Juan Carlos, Móstoles, Spain.
  • Mahillo I; Cytek Biosciences Inc., Fremont, California.
  • Cabello A; Grupo de respuesta inmune innata, IdiPAZ, Hospital Universitario La Paz, Madrid, Spain.
  • López-Collazo E; Department of Statistics, Instituto de Investigación Sanitaria Fundación Jiménez Díaz, Universidad Autónoma de Madrid (IIS-FJD, UAM), Madrid, Spain.
  • Sánchez-Cabo F; Hospital Universitario Fundación Jiménez Díaz, Madrid, Spain.
  • Górgolas M; Grupo de respuesta inmune innata, IdiPAZ, Hospital Universitario La Paz, Madrid, Spain.
  • Estrada V; Unidad de Bioinformática, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
  • Benito JM; Hospital Universitario Fundación Jiménez Díaz, Madrid, Spain.
J Med Virol ; 96(5): e29646, 2024 May.
Article em En | MEDLINE | ID: mdl-38699988
ABSTRACT
Elite controllers (ECs) are an exceptional group of people living with HIV (PLWH) that control HIV replication without therapy. Among the mechanisms involved in this ability, natural killer (NK)-cells have recently gained much attention. We performed an in-deep phenotypic analysis of NK-cells to search for surrogate markers associated with the long term spontaneous control of HIV. Forty-seven PLWH (22 long-term EC [PLWH-long-term elite controllers (LTECs)], 15 noncontrollers receiving antiretroviral treatment [ART] [PLWH-onART], and 10 noncontrollers cART-naïve [PLWH-offART]), and 20 uninfected controls were included. NK-cells homeostasis was analyzed by spectral flow cytometry using a panel of 15 different markers. Data were analyzed using FCSExpress and R software for unsupervised multidimensional analysis. Six different subsets of NK-cells were defined on the basis of CD16 and CD56 expression, and the multidimensional analysis revealed the existence of 68 different NK-cells clusters based on the expression levels of the 15 different markers. PLWH-offART presented the highest disturbance of NK-cells homeostasis and this was not completely restored by long-term ART. Interestingly, long term spontaneous control of HIV (PLWH-LTEC group) was associated with a specific profile of NK-cells homeostasis disturbance, characterized by an increase of CD16dimCD56dim subset when compared to uninfected controls (UC) group and also to offART and onART groups (p < 0.0001 for the global comparison), an increase of clusters C16 and C26 when compared to UC and onART groups (adjusted p-value < 0.05 for both comparisons), and a decrease of clusters C10 and C20 when compared to all the other groups (adjusted p-value < 0.05 for all comparisons). These findings may provide clues to elucidate markers of innate immunity with a relevant role in the long-term control of HIV.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Matadoras Naturais / Infecções por HIV Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Matadoras Naturais / Infecções por HIV Idioma: En Ano de publicação: 2024 Tipo de documento: Article