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Massive field-of-view sub-cellular traction force videography enabled by Single-Pixel Optical Tracers (SPOT).
Marvin Tan, Xing Haw; Wang, Yijie; Zhu, Xiongfeng; Mendes, Felipe Nanni; Chung, Pei-Shan; Chow, Yu Ting; Man, Tianxing; Lan, Hsin; Lin, Yen-Ju; Zhang, Xiang; Zhang, Xiaohe; Nguyen, Thang; Ardehali, Reza; Teitell, Michael A; Deb, Arjun; Chiou, Pei-Yu.
Afiliação
  • Marvin Tan XH; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Bioengineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Electronics and Ph
  • Wang Y; Division of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, 675 Charles E Young Dr S, Los Angeles, CA, 90095, United States.
  • Zhu X; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Mendes FN; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Chung PS; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Bioengineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Chow YT; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Man T; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Lan H; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Lin YJ; Department of Electrical and Computer Engineering, University of California at Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Zhang X; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Zhang X; Department of Mathematics, University of California Los Angeles, 520 Portola Plaza, Los Angeles, CA, 90095, United States.
  • Nguyen T; Department of Bioengineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States.
  • Ardehali R; Division of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, 675 Charles E Young Dr S, Los Angeles, CA, 90095, United States.
  • Teitell MA; Department of Bioengineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, 675 Charles E Young Dr S, Los Angeles, CA, 90095, United
  • Deb A; Division of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, 675 Charles E Young Dr S, Los Angeles, CA, 90095, United States.
  • Chiou PY; Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Bioengineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States. Electronic address: peiyu@g.ucla
Biosens Bioelectron ; 258: 116318, 2024 Aug 15.
Article em En | MEDLINE | ID: mdl-38701538
ABSTRACT
We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm2 at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Miócitos Cardíacos Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Miócitos Cardíacos Idioma: En Ano de publicação: 2024 Tipo de documento: Article