Structural elucidation of the mesothelin-mucin-16/CA125 interaction.
Structure
; 32(8): 1049-1054.e2, 2024 Aug 08.
Article
em En
| MEDLINE
| ID: mdl-38703776
ABSTRACT
Mesothelin (MSLN) is a cell-surface glycoprotein expressed at low levels on normal mesothelium but overexpressed in many cancers. Mesothelin has been implicated to play role/s in cell adhesion and multiple signaling pathways. Mucin-16/CA125 is an enormous cell-surface glycoprotein, also normally expressed on mesothelium and implicated in the progression and metastasis of several cancers, and directly binds mesothelin. However, the precise biological function/s of mesothelin and mucin-16/CA125 remain mysterious. We report protein engineering and recombinant production, qualitative and quantitative binding studies, and a crystal structure determination elucidating the molecular-level details governing recognition of mesothelin by mucin-16/CA125. The interface is small, consistent with the â¼micromolar binding constant and is free of glycan-mediated interactions. Sequence comparisons and modeling suggest that multiple mucin-16/CA125 modules can interact with mesothelin through comparable interactions, potentially generating a high degree of avidity at the cell surface to overcome the weak affinity, with implications for functioning and therapeutic interventions.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ligação Proteica
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Modelos Moleculares
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Antígeno Ca-125
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Proteínas Ligadas por GPI
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Mesotelina
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article