[Study of antitumor effects of human placenta hydrolysate on PC-3, OAW-42, BT-474 cell cultures].
Ter Arkh
; 96(3): 266-272, 2024 Apr 16.
Article
em Ru
| MEDLINE
| ID: mdl-38713042
ABSTRACT
AIM:
To investigate the antitumor effects of human placenta hydrolysate (HPH) peptides on three hormone-dependent human cell lines prostate adenocarcinoma, breast carcinoma, and ovarian cancer by metabolic analysis of cell cultures. MATERIALS ANDMETHODS:
The effect of HPH on tumor and control tumor cell lines was evaluated. Study stages (A) de novo peptide sequencing by collision-induced dissociation mass spectrometry; (B) detection of peptides with anti-tumor properties; (C) expert analysis of the obtained lists of peptides.RESULTS:
Dose-dependent cytotoxic effects of HPH on three tumor cell lines are shown PC-3 (human prostate adenocarcinomas), OAW-42 (human ovarian cancer), BT-474 (human breast carcinomas), and IC50 constants (1.3-2.8 mg/ml) were obtained. The analysis of the HPH peptide fraction showed more than 70 peptides with antitumor properties in the composition of this HPH, including kinase inhibitors mitogen-activated protein kinases, kappa-bi nuclear factor inhibitor kinase, AKT serine/threonine kinase 1, protein kinase C zeta, interleukin-1 receptor-associated kinase 4 and cyclin-dependent kinase 1.CONCLUSION:
The results of the study indicate not only the oncological safety of the HPH used in therapy but also the mild antitumor effects of this HPH at high concentrations.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Neoplasias da Próstata
/
Neoplasias da Mama
Idioma:
Ru
Ano de publicação:
2024
Tipo de documento:
Article