Sphingobacterium oryzagri sp. nov., isolated from rice paddy soil.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
em En
| MEDLINE
| ID: mdl-38713185
ABSTRACT
An aerobic, Gram-stain-negative and short rod-shaped bacterial strain, designated M6-31T, was isolated from rice paddy soil sampled in Miryang, Republic of Korea. Growth was observed at 4-35â°C (optimum, 28â°C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-4â% (w/v) NaCl (optimum, 0â% w/v). Phylogenetic analysis based on 16S rRNA gene sequences grouped strain M6-31T with Sphingobacterium bambusae IBFC2009T, Sphingobacterium griseoflavum SCU-B140T and Sphingobacterium solani MLS-26-JM13-11T in the same clade, with the 16S rRNA gene sequence similarities ranging from 95.8 to 96.6â%. A genome-based phylogenetic tree reconstructed by using all publicly available Sphingobacterium genomes placed strain M6-31T with S. bambusae KACC 22910T, 'Sphingobacterium deserti' ACCC 05744T, S. griseoflavum CGMCC 1.12966T and Sphingobacterium paludis CGMCC 1.12801T. Orthologous average nucleotide identity and digital DNA-DNA hybridization values between strain M6-31T and its closely related strains were lower than 74.6 and 22.0â%, respectively. The respiratory quinone was menaquinone-7, and the major polar lipid was phosphatidylethanolamine. The major fatty acids (>10â%) were C15ââ0 iso, C17ââ0 iso 3OH and summed feature 3. The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that strain M6-31T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium oryzagri sp. nov. (type strain M6-31T=KACC 22765T=JCM 35893T) is proposed.
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MEDLINE
Assunto principal:
Filogenia
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Oryza
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Microbiologia do Solo
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Vitamina K 2
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Sphingobacterium
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Ácidos Graxos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article