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Quantum Sensing of Free Radical Generation in Mitochondria of Human Keratinocytes during UVB Exposure.
Fan, Siyu; Lopez Llorens, Lluna; Perona Martinez, Felipe P; Schirhagl, Romana.
Afiliação
  • Fan S; Department of Biomaterials & Biomedical Technology, University Medical Center Groningen, University Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
  • Lopez Llorens L; Department of Biomaterials & Biomedical Technology, University Medical Center Groningen, University Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
  • Perona Martinez FP; Department of Biomaterials & Biomedical Technology, University Medical Center Groningen, University Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
  • Schirhagl R; Department of Biomaterials & Biomedical Technology, University Medical Center Groningen, University Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
ACS Sens ; 9(5): 2440-2446, 2024 05 24.
Article em En | MEDLINE | ID: mdl-38743437
ABSTRACT
Ultraviolet (UV) radiation is known to cause skin issues, such as dryness, aging, and even cancer. Among UV rays, UVB stands out for its ability to trigger problems within cells, including mitochondrial dysfunction, oxidative stress, and DNA damage. Free radicals are implicated in these cellular responses, but they are challenging to measure due to their short lifetime and limited diffusion range. In our study, we used a quantum sensing technique (T1 relaxometry) involving fluorescent nanodiamonds (FNDs) that change their optical properties in response to magnetic noise. This allowed us to monitor the free radical presence in real time. To measure radicals near mitochondria, we coated FNDs with antibodies, targeting mitochondrial protein voltage-dependent anion channel 2 (anti-VDAC2). Our findings revealed a dynamic rise in radical levels on the mitochondrial membrane as cells were exposed to UVB (3 J/cm2), with a significant increase observed after 17 min.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Queratinócitos / Mitocôndrias Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Queratinócitos / Mitocôndrias Idioma: En Ano de publicação: 2024 Tipo de documento: Article