Controllable concentric electric field line distribution for simultaneous separation of DNA.
J Chromatogr A
; 1727: 464990, 2024 Jul 19.
Article
em En
| MEDLINE
| ID: mdl-38744188
ABSTRACT
An approach for the controllable separation and concentration of nucleic acid using a circular nonuniform electric field was proposed and developed. Using six different lengths of DNA molecules as standard samples, the distribution of the gradient electric field was increased from the outer circular electrode to the inner rod-shaped electrode, contributing to the migration of DNA molecules at a velocity gradient towards the region with the strongest inner electric field. The DNA molecules were arranged in a distribution of concentric circles that aligned with the distribution of concentric equipotential lines. The concentration of DNA multiplied with the alternation of radius. As a result, this platform allowed simultaneous DNA separation, achieving a resolution range of 1.17-3.03 through an extended electrophoresis time, resulting in enhanced concentration factors of 1.08-6.27. Moreover, the manipulation of the relative height of the inner and outer electrodes enabled precise control over the distribution and the deflection degree of electric field lines, leading to accurate control over DNA deflection.
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Base de dados:
MEDLINE
Assunto principal:
DNA
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article