An investigation into the effect of reagent volume on the spin indirect antiglobulin test.
Vox Sang
; 49(2): 114-20, 1985.
Article
em En
| MEDLINE
| ID: mdl-3875931
ABSTRACT
Three polyspecific antiglobulin reagents were tested in a spin antiglobulin technique with 51 antibodies of varying blood group specificities in which the volume of antiglobulin reagent used ranged from 1 volume (35 microliters) to 3 volumes (105 microliters). Statistical analysis of results showed that a significant decrease in avidity and agglutination scores occurred as the volume of antiglobulin reagent used was increased ('volume effect'). The volume effect was shown by 33/51 (65%) of antibodies with all three antiglobulin reagents and by 48/51 (94%) of antibodies with at least one antiglobulin reagent. Only 3/51 (6%) failed to show the volume effect with all three antiglobulin reagents.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Teste de Coombs
/
Anticorpos Anti-Idiotípicos
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article