Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.
Elife
; 132024 May 22.
Article
em En
| MEDLINE
| ID: mdl-38775133
ABSTRACT
Tissue-clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. HIF-Clear with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain and is able to be used as the evaluation of disease treatment efficiency. HIF-Clear also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen, as well as a 3-month formalin-fixed mouse brain. Thus, HIF-Clear represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.
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Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Fixação de Tecidos
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Inclusão em Parafina
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Formaldeído
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Neurônios
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article