Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS.
Food Chem
; 453: 139667, 2024 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-38776794
ABSTRACT
B-group vitamins are important micronutrients for maintaining human health; nevertheless, B vitamin deficiency is a globally widespread issue. Thus, it is relevant to accurately assess the B-vitamin content in staple crop products such as wheat grains. Here, we developed a multi-enzyme extraction method allowing accurate quantification of seven B vitamins in wheat using ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Free forms of thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7) and folates (B9) were determined with recoveries ranging from 81 to 118% and accuracy below 15% bias. The precision was below 20% relative standard deviation and the internal standards adequately compensated for matrix effects. The method was applied to determine the B vitamin stabilities in wheat grains stored at different temperatures and periods. The results provide an important basis in future studies aiming at understanding nutritional availability of B vitamins.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexo Vitamínico B
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Triticum
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Espectrometria de Massas em Tandem
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article