Structural and functional insights in flavivirus NS5 proteins gained by the structure of Ntaya virus polymerase and methyltransferase.
Structure
; 32(8): 1099-1109.e3, 2024 Aug 08.
Article
em En
| MEDLINE
| ID: mdl-38781970
ABSTRACT
Flaviviruses are single-stranded positive-sense RNA (+RNA) viruses that are responsible for several (re)emerging diseases such as yellow, dengue, or West Nile fevers. The Zika epidemic highlighted their dangerousness when a relatively benign virus known since the 1950s turned into a deadly pathogen. The central protein for their replication is NS5 (non-structural protein 5), which is composed of the N-terminal methyltransferase (MTase) domain and the C-terminal RNA-dependent RNA-polymerase (RdRp) domain. It is responsible for both RNA replication and installation of the 5' RNA cap. We structurally and biochemically analyzed the Ntaya virus MTase and RdRp domains and we compared their properties to other flaviviral NS5s. The enzymatic centers are well conserved across Flaviviridae, suggesting that the development of drugs targeting all flaviviruses is feasible. However, the enzymatic activities of the isolated proteins were significantly different for the MTase domains.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Polimerase Dependente de RNA
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Modelos Moleculares
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Proteínas não Estruturais Virais
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Metiltransferases
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article