Your browser doesn't support javascript.
loading
Isolation and molecular identification of Leptospira santarosai and Leptospira interrogans in equines from eastern Mexico.
Ramos-Vázquez, José R; Sánchez-Montes, Sokani; Esparza-González, Sandra C; Romero-Salas, Dora; Pardio-Sedas, Violeta; Estrada-Coates, Alejandro T; Alva-Trujillo, Miriam; Ballados-González, Gerardo G; Grostieta, Estefanía; Becker, Ingeborg; Cruz-Romero, Anabel.
Afiliação
  • Ramos-Vázquez JR; Laboratorio de Enfermedades Infecciosas, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.
  • Sánchez-Montes S; Laboratorio de Diagnóstico, Facultad de Ciencias Biológicas y Agropecuarias, región Poza Rica-Tuxpan, Universidad Veracruzana, Tuxpan de Rodríguez Cano, Veracruz, Mexico; Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de
  • Esparza-González SC; Facultad de Odontología Unidad Saltillo, Universidad Autónoma de Coahuila. Saltillo, Mexico.
  • Romero-Salas D; Laboratorio de Parasitología, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.
  • Pardio-Sedas V; Laboratorio de Seguridad Agroalimentaria, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.
  • Estrada-Coates AT; Hospital de Grandes Especies, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.
  • Alva-Trujillo M; Hospital de Grandes Especies, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.
  • Ballados-González GG; Laboratorio de Enfermedades Infecciosas, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico.
  • Grostieta E; Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
  • Becker I; Centro de Medicina Tropical, División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
  • Cruz-Romero A; Laboratorio de Enfermedades Infecciosas, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz, Mexico. Electronic address: anabcruz@uv.mx.
Acta Trop ; 256: 107242, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38782111
ABSTRACT
Leptospirosis is an infectious disease with a worldwide distribution, which represents a major challenge in animal production across developing countries, mainly in tropical areas. Horses are particularly susceptible to the disease, presenting manifestations ranging from subclinical to the development of uveitis that compromises the visual health of the animals. In recent years, serological studies have been carried out in equid populations from America, demonstrating high exposure. For this reason, the aim of this study was to demonstrate microbiologically and molecularly the presence of the members of the genus Leptospira in urine samples from equids in an endemic state of leptospirosis in Mexico, and to detect the serological presence of anti-Leptospira antibodies in the sampled animals. For this reason, blood and urine samples were collected from 28 horses and one mule from three localities in the state of Veracruz, Mexico. Urine samples were inoculated in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, and the recovered isolates were typed using a short Multi Locus Sequence Typing scheme. Amplifications of the expected size were subjected to sequencing, and the recovered sequences were compared with those of reference deposited in GenBank using the BLAST tool. To identify their phylogenetic position, we performed a phylogenetic reconstruction using the maximum likelihood method. Additionally, Microscopic Agglutination test was performed on the serum samples to identify anti-Leptospira antibodies. We recovered 16 urine isolates which tested positive for the presence of Leptospira DNA. The phylogenetic reconstruction and the MLST analysis confirmed the presence of several genotypes of Leptospira interrogans and Leptospira santarosai. An overall serological frequency of 97.1 % was detected. Our results represent the first record of the presence of Leptospira through bacteriological isolates in equids from Mexico.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Doenças dos Cavalos / Leptospira / Leptospirose / Anticorpos Antibacterianos Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Filogenia / Doenças dos Cavalos / Leptospira / Leptospirose / Anticorpos Antibacterianos Idioma: En Ano de publicação: 2024 Tipo de documento: Article