Heterochromatic 3D genome organization is directed by HP1a- and H3K9-dependent and independent mechanisms.
Mol Cell
; 84(11): 2017-2035.e6, 2024 Jun 06.
Article
em En
| MEDLINE
| ID: mdl-38795706
ABSTRACT
Whether and how histone post-translational modifications and the proteins that bind them drive 3D genome organization remains unanswered. Here, we evaluate the contribution of H3K9-methylated constitutive heterochromatin to 3D genome organization in Drosophila tissues. We find that the predominant organizational feature of wild-type tissues is the segregation of euchromatic chromosome arms from heterochromatic pericentromeres. Reciprocal perturbation of HP1aâ
H3K9me binding, using a point mutation in the HP1a chromodomain or replacement of the replication-dependent histone H3 with H3K9R mutant histones, revealed that HP1a binding to methylated H3K9 in constitutive heterochromatin is required to limit contact frequency between pericentromeres and chromosome arms and regulate the distance between arm and pericentromeric regions. Surprisingly, the self-association of pericentromeric regions is largely preserved despite the loss of H3K9 methylation and HP1a occupancy. Thus, the HP1aâ
H3K9 interaction contributes to but does not solely drive the segregation of euchromatin and heterochromatin inside the nucleus.
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Assunto principal:
Proteínas Cromossômicas não Histona
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Heterocromatina
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Histonas
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Proteínas de Drosophila
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Drosophila melanogaster
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Homólogo 5 da Proteína Cromobox
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article