10× single-cell sequencing revealed cellular composition heterogeneity in cardiac myxoma with malignant glandular properties.
Microvasc Res
; 154: 104697, 2024 07.
Article
em En
| MEDLINE
| ID: mdl-38801942
ABSTRACT
Cardiac myxoma is the most common primary cardiac tumor in adults. The histogenesis and cellular composition of myxoma are still unclear. This study aims to reveal the role of myxoma cell components and their gene expression in tumor development. We obtained single living cells by enzymatic digestion of tissues from 4 cases of surgically resected cardiac myxoma. Of course, there was 1 case of glandular myxoma and 3 cases of nonglandular myxoma. Then, 10× single-cell sequencing was performed. We identified 12 types and 11 types of cell populations in glandular myxoma and nonglandular myxoma, respectively. Heterogeneous epithelial cells are the main components of glandular myxoma. The similarities and differences in T cells in both glandular and nonglandular myxoma were analyzed by KEGG and GO. The most important finding was that there was active communication between T cells and epithelial cells. These results clarify the possible tissue occurrence and heterogeneity of cardiac myxoma and provide a theoretical basis and guidance for clinical diagnosis and treatment.
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Base de dados:
MEDLINE
Assunto principal:
Análise de Célula Única
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Neoplasias Cardíacas
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Mixoma
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article