Protocol for generation of and high-throughput drug testing with patient-derived colorectal cancer organoids.
STAR Protoc
; 5(2): 103090, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38809757
ABSTRACT
Drug sensitivity testing of patient-derived tumor organoids (PDTOs) is a promising tool for personalizing cancer treatment. Here, we present a protocol for generation of and high-throughput drug testing with PDTOs. We describe detailed steps for PDTO establishment from colorectal cancer tissues, preparation of PDTOs for high-throughput drug testing, and quantification of drug testing results using image analysis. This protocol provides a standardized workflow for PDTO testing of standard-of-care therapies, along with exploring the activity of new agents, for translational research. For complete details on the use and execution of this protocol, please refer to Tan et al.1.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ensaios de Seleção de Medicamentos Antitumorais
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Neoplasias Colorretais
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Organoides
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Ensaios de Triagem em Larga Escala
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article