FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo.
Mol Cell
; 84(11): 2053-2069.e9, 2024 Jun 06.
Article
em En
| MEDLINE
| ID: mdl-38810649
ABSTRACT
Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription through chromatin in vitro, but its functional roles in vivo remain unclear. Here, we analyze the in vivo functions of FACT with the use of multi-omics analysis after rapid FACT depletion from human cells. We show that FACT depletion destabilizes chromatin and leads to transcriptional defects, including defective promoter-proximal pausing and elongation, and increased premature termination of RNA polymerase II. Unexpectedly, our analysis revealed that promoter-proximal pausing depends not only on the negative elongation factor (NELF) but also on the +1 nucleosome, which is maintained by FACT.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
RNA Polimerase II
/
Cromatina
/
Proteínas de Grupo de Alta Mobilidade
/
Nucleossomos
/
Regiões Promotoras Genéticas
/
Fatores de Elongação da Transcrição
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article