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MIR396-GRF/GIF enhances in planta shoot regeneration of Dendrobium catenatum.
Yang, Zhenyu; Zhao, Meili; Zhang, Xiaojie; Gu, Lili; Li, Jian; Ming, Feng; Wang, Meina; Wang, Zhicai.
Afiliação
  • Yang Z; Shenzhen Key Laboratory for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen, 518114, China.
  • Zhao M; Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen, 518114, China.
  • Zhang X; Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
  • Gu L; Shenzhen Key Laboratory for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen, 518114, China.
  • Li J; Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen, 518114, China.
  • Ming F; Shenzhen Key Laboratory for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen, 518114, China.
  • Wang M; Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, the National Orchid Conservation Center of China and the Orchid Conservation & Research Center of Shenzhen, Shenzhen, 518114, China.
  • Wang Z; Xinjiang Key Laboratory of Grassland Resources and Ecology, College of Grassland Sciences, Xinjiang Agricultural University, Urumqi, 830052, China.
BMC Genomics ; 25(1): 543, 2024 May 31.
Article em En | MEDLINE | ID: mdl-38822270
ABSTRACT
Recent studies on co-transformation of the growth regulator, TaGRF4-GIF1 chimera (Growth Regulating Factor 4-GRF Interacting Factor 1), in cultivated wheat varieties (Triticum aestivum), showed improved regeneration efficiency, marking a significant breakthrough. Here, a simple and reproducible protocol using the GRF4-GIF1 chimera was established and tested in the medicinal orchid Dendrobium catenatum, a monocot orchid species. TaGRF4-GIF1 from T. aestivum and DcGRF4-GIF1 from D. catenatum were reconstructed, with the chimeras significantly enhancing the regeneration efficiency of D. catenatum through in planta transformation. Further, mutating the microRNA396 (miR396) target sites in TaGRF4 and DcGRF4 improved regeneration efficiency. The target mimicry version of miR396 (MIM396) not only boosted shoot regeneration but also enhanced plant growth. Our methods revealed a powerful tool for the enhanced regeneration and genetic transformation of D. catenatum.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regeneração / Brotos de Planta / Dendrobium / MicroRNAs Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Regeneração / Brotos de Planta / Dendrobium / MicroRNAs Idioma: En Ano de publicação: 2024 Tipo de documento: Article