Functional Role of C-terminal Domains in the MSL2 Protein of Drosophila melanogaster.
Biochemistry (Mosc)
; 89(4): 663-673, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38831503
ABSTRACT
Dosage compensation complex (DCC), which consists of five proteins and two non-coding RNAs roX, specifically binds to the X chromosome in males, providing a higher level of gene expression necessary to compensate for the monosomy of the sex chromosome in male Drosophila compared to the two X chromosomes in females. The MSL2 protein contains the N-terminal RING domain, which acts as an E3 ligase in ubiquitination of proteins and is the only subunit of the complex expressed only in males. Functional role of the two C-terminal domains of the MSL2 protein, enriched with proline (P-domain) and basic amino acids (B-domain), was investigated. As a result, it was shown that the B-domain destabilizes the MSL2 protein, which is associated with the presence of two lysines ubiquitination of which is under control of the RING domain of MSL2. The unstructured proline-rich domain stimulates transcription of the roX2 gene, which is necessary for effective formation of the dosage compensation complex.
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MEDLINE
Assunto principal:
Proteínas de Drosophila
/
Drosophila melanogaster
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Domínios Proteicos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article