HIV-1 virion lysis following centrifugation improves the sensitivity of the Fourth-Generation HIV Ag/Ab combo assay.
BMC Res Notes
; 17(1): 153, 2024 Jun 04.
Article
em En
| MEDLINE
| ID: mdl-38835056
ABSTRACT
OBJECTIVE:
Fourth-generation HIV Ag/Ab Combo assay is used for HIV screening of blood for transfusion in developing countries, however, the sensitivity of the assay is questionable during the acute phase of HIV infection. Thus, the study aimed to determine the effect of combining centrifugation with HIV-1 virion lysis on the sensitivity of the fourth-generation HIV Ag/Ab combo assay.RESULTS:
When the 50 HIV-1 antibody-negative samples were run on the fourth-generation HIV Ag/Ab combo assay, 8 (16%) were positive following centrifugation, 13 (26%) were positive following lysis while 25 (50%) were positive after combining centrifugation with HIV-1 virion lysis.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Vírion
/
Anticorpos Anti-HIV
/
Infecções por HIV
/
Centrifugação
/
Sensibilidade e Especificidade
/
HIV-1
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article