Gallic acid pretreatment mitigates parathyroid ischemia-reperfusion injury through signaling pathway modulation.
Sci Rep
; 14(1): 12971, 2024 06 05.
Article
em En
| MEDLINE
| ID: mdl-38839854
ABSTRACT
Thyroid surgery often results in ischemia-reperfusion injury (IRI) to the parathyroid glands, yet the mechanisms underlying this and how to ameliorate IRI remain incompletely explored. Our study identifies a polyphenolic herbal extract-gallic acid (GA)-with antioxidative properties against IRI. Through flow cytometry and CCK8 assays, we investigate the protective effects of GA pretreatment on a parathyroid IRI model and decode its potential mechanisms via RNA-seq and bioinformatics analysis. Results reveal increased apoptosis, pronounced G1 phase arrest, and significantly reduced cell proliferation in the hypoxia/reoxygenation group compared to the hypoxia group, which GA pretreatment mitigates. RNA-seq and bioinformatics analysis indicate GA's modulation of various signaling pathways, including IL-17, AMPK, MAPK, transient receptor potential channels, cAMP, and Rap1. In summary, GA pretreatment demonstrates potential in protecting parathyroid cells from IRI by influencing various genes and signaling pathways. These findings offer a promising therapeutic strategy for hypoparathyroidism treatment.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Glândulas Paratireoides
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Traumatismo por Reperfusão
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Transdução de Sinais
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Apoptose
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Ácido Gálico
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article