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The long noncoding RNA SUMO1P3 as urinary biomarker for monitoring bladder cancer progression.
Galbiati, Silvia; Bettiga, Arianna; Colciago, Giorgia; Senti, Chiara; Trevisani, Francesco; Villa, Giulia; Marzinotto, Ilaria; Ghidini, Michele; Passalacqua, Rodolfo; Montorsi, Francesco; Salonia, Andrea; Vago, Riccardo.
Afiliação
  • Galbiati S; Complications of Diabetes Unit, Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Bettiga A; Urological Research Institute, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Colciago G; Urological Research Institute, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Senti C; Oncology Unit, ASST of Cremona, Hospital of Cremona, Cremona, Italy.
  • Trevisani F; Urological Research Institute, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Villa G; Center for Nursing Research and Innovation, Vita-Salute San Raffaele University, Milan, Italy.
  • Marzinotto I; Beta Cell Biology Unit, Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Ghidini M; Oncology Unit, ASST of Cremona, Hospital of Cremona, Cremona, Italy.
  • Passalacqua R; Oncology Unit, ASST of Cremona, Hospital of Cremona, Cremona, Italy.
  • Montorsi F; Urological Research Institute, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
  • Salonia A; Faculty of Medicine and Surgery, Università Vita-Salute San Raffaele, Milan, Italy.
  • Vago R; Urological Research Institute, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Front Oncol ; 14: 1325157, 2024.
Article em En | MEDLINE | ID: mdl-38846969
ABSTRACT

Introduction:

Urothelial Bladder Cancer (BC) is the ninth most common cancer worldwide. It is classified into Non Muscle Invasive (NMIBC) and Muscle Invasive Bladder Cancer (MIBC), which are characterized by frequent recurrences and progression rate, respectively. The diagnosis and monitoring are obtained through invasive methods as cystoscopy and post-surgery biopsies. Thus, a panel of biomarkers able to discriminate BC based on grading or staging represents a significant step forward in the patients' workup. In this perspective, long non-coding RNAs (lncRNAs) are emerged as reliable candidates as potential biomarker given their specific and regulated expression. In the present work we propose two lncRNAs, the Small Ubiquitin Modifier 1 pseudogene 3 (SUMO1P3), a poorly characterized pseudogene, and the Urothelial Carcinoma Associated 1 (UCA1) as candidates to monitor the BC progression.

Methods:

This study was a retrospective trial enrolling NMIBC and MIBC patients undergoing surgical intervention the expression of the lncRNA SUMO1P3 and UCA1 was evaluated in urine from 113 subjects (cases and controls). The receiver operating characteristic curve analysis was used to evaluate the performance of single or combined biomarkers in discriminating cases from controls.

Results:

SUMO1P3 and UCA1 expression in urine was able to significantly discriminate low grade NMIBC, healthy control and benign prostatic hyperplasia subjects versus high grade NMIBC and MIBC patients. We also demonstrated that miR-320a, which binds SUMO1P3, was reduced in high grade NMIBC and MIBC patients and the SUMO1P3/miR-320a ratio was used to differentiate cases versus controls, showing a statistically significant power. Finally, we provided an automated method of RNA extraction coupled to ddPCR analysis in a perspective of clinical application.

Discussion:

We have shown that the lncRNA SUMO1P3 is increased in urine from patients with high grade NMIBC and MIBC and that it is likely to be good candidate to predict bladder cancer progression if used alone or in combination with UCA1 or with miRNA320a.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article