Quantitative analysis of anthocyanins and flavonoids in purple and green leaves of Eleutherococcus senticosus by UPLC-MS/MS techniques.

ABSTRACT

Ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-MS/MS) were used to target quantitative determination anthocyanins and flavonoids in the fresh leaves (purple and green) of Eleutherococcus senticosus. The results showed that the content of total anthocyanins was 99.68 µg/g (Fresh Weight, FW) in purple leaves and 29.12 µg/g in green leaves. Cyanidin-3-O-galactoside and delphinidin were the main anthocyanins compound in purple and green leaves, and the content of the both declined sharply in green leaves. The content of cyanidin-3-O-galactoside reached 616.23 ng/100 mg in purple leaves and was only fifth in green leaves. The total flavonoids content was 4.90 mg/g in purple leaves and 2.23 mg/g in green leaves. Quercetin-3-ß-D-glucoside (236.96 ng/mg) and kaempferol-3-O-glucoside (145.27 ng/mg) were the main flavonoids compound in purple leaves. Besides the two main flavonoids, large quantities of rutin (269.11 ng/mg) was detected in green leaves of E. senticosus.