Protocol for Seahorse analysis of ex vivo mouse brown and white adipose tissues.
STAR Protoc
; 5(2): 103042, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38850537
ABSTRACT
The mitochondrial stress test is a gold-standard approach for assessing adipose tissue physiological functions and pathological changes. Here, we present a protocol for conducting Seahorse assays using ex vivo mouse brown and white adipose depots. We describe steps for rehydrating the cartridge, preparing freshly harvested fat depots, placing them onto an islet capture plate, and incubating them in a non-CO2 incubator. We then detail procedures for adding mitochondrial stressor solutions and conducting the mitochondrial stress test using the Seahorse XFe24 Analyzer. For complete details on the use and execution of this protocol, please refer to An et al.1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo Marrom
/
Tecido Adiposo Branco
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article