Construction and evaluation of AuNPs enhanced electrochemical immunosensors with [Fe(CN)6]3-/4- and PPy probe for highly sensitive detection of human chorionic gonadotropin.
Int J Biol Macromol
; 273(Pt 1): 132963, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38852725
ABSTRACT
Human chorionic gonadotropin (HCG), a vital protein for pregnancy determination and a marker for trophoblastic diseases, finds application in monitoring early pregnancy and ectopic pregnancy. This study presents an innovative approach employing electrochemical immunosensors for enhanced HCG detection, utilizing Anti-HCG antibodies and gold nanoparticles (AuNPs) in the sensor platform. Two sensor configurations were optimized BSA/Anti-HCG/c-AuNPs/MEL/e-AuNPs/SPCE with [Fe(CN)6]3-/4- as a redox probe (1) and BSA/Anti-HCG/PPy/e-AuNPs/SPCE using polypyrrole (PPy) as a redox probe (2). The first sensor offers linear correlation in the 0.10-500.00 pgâmL-1 HCG range, with a limit of detection (LOD) of 0.06 pgâmL-1, sensitivity of 32.25 µAâpg-1âmLâcm-2, RSD <2.47 %, and a recovery rate of 101.03-104.81 %. The second sensor widens the HCG detection range (40.00 fgâmL-1-5.00 pgâmL-1) with a LOD of 16.53 fgâmL-1, ensuring precision (RSD <1.04 %) and a recovery range of 94.61-106.07 % in serum samples. These electrochemical immunosensors have transformative potential in biomarker detection, offering enhanced sensitivity, selectivity, and stability for advanced healthcare diagnostics.
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Base de dados:
MEDLINE
Assunto principal:
Polímeros
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Pirróis
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Técnicas Biossensoriais
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Nanopartículas Metálicas
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Técnicas Eletroquímicas
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Limite de Detecção
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Ouro
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Gonadotropina Coriônica
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article