First laboratory-confirmed case of scrub typhus in Shijiazhuang City, Hebei Province.
Front Microbiol
; 15: 1409949, 2024.
Article
em En
| MEDLINE
| ID: mdl-38855771
ABSTRACT
Objective:
Defining whether a suspected case was due to scrub typhus through laboratory testing, to understand the prevalence of scrub typhus in Shijiazhuang City, Hebei Province.Methods:
An epidemiological investigation was conducted on the suspected case, utilizing Weil-Felix test and indirect immunofluorescence assay (IFA) to detect specific antibodies against O. tsutsugamushi in serum specimens. Additionally, PCR amplification of the 56-kDa and groEL genes was performed, followed by constructing a phylogenetic tree to identify the genotype.Results:
The acute phase titer of the Weil-Felix test for the case was 1160, which increased to 1320 in the recovery phase. IFA assay revealed IgG titers against O. tsutsugamushi of 164 in the acute phase and 1256 in the recovery phase. Sequence alignment of the PCR amplified fragment showed the highest similarity with the O. tsutsugamushi genotype. Kawasaki sequence, ranging from 99.71 to 100.00%. The strain exhibited the closest genetic relationship with the known O. tsutsugamushi Kawasaki genotype.Conclusion:
This study confirms the presence of O. tsutsugamushi in Shijiazhuang City, Hebei Province, with the identified strain belonging to the Kawasaki genotype, marking the first diagnosis of this strain in the region.
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article