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Progesterone and 17-hydroxy-progesterone concentrations in follicular fluid and serum reflect their production in granulosa and theca cells.
Zheng, M; Poulsen, L C; Wang, N F; Mamsen, L S; Johannsen, M L; Styrishave, B; Grøndahl, M L; Løssl, K; Englund, A L M; Skouby, S O; Andersen, C Y.
Afiliação
  • Zheng M; Laboratory of Reproductive Biology, Copenhagen University Hospital, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark. Electronic address: mengxue.zheng@regionh.dk.
  • Poulsen LC; The Fertility Clinic, Herlev Hospital, Herlev, Denmark.
  • Wang NF; The Fertility Clinic, Copenhagen University Hospital, Copenhagen, Denmark.
  • Mamsen LS; Laboratory of Reproductive Biology, Copenhagen University Hospital, Copenhagen, Denmark.
  • Johannsen ML; Laboratory of Reproductive Biology, Copenhagen University Hospital, Copenhagen, Denmark; Toxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Styrishave B; Toxicology and Drug Metabolism Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Grøndahl ML; The Fertility Clinic, Herlev Hospital, Herlev, Denmark.
  • Løssl K; The Fertility Clinic, Copenhagen University Hospital, Copenhagen, Denmark.
  • Englund ALM; The Fertility Clinic, Zealand University Hospital, Køge, Denmark.
  • Skouby SO; The Fertility Clinic, Herlev Hospital, Herlev, Denmark.
  • Andersen CY; Department of Clinical Medicine, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark; The Fertility Clinic, Herlev Hospital, Herlev, Denmark. Electronic address: cya@yding.com.
Reprod Biomed Online ; 49(2): 103853, 2024 08.
Article em En | MEDLINE | ID: mdl-38865783
ABSTRACT
RESEARCH QUESTION How is the production of progesterone (P4) and 17-hydroxy-P4 (17-OH-P4) regulated between theca cells and granulosa cells during the follicular phase, during ovulation and after transformation into a corpus luteum?

DESIGN:

Three cohorts were examined (i) 31 women undergoing natural and stimulated cycles, with serum hormone measurements taken every 3 days; (ii) 50 women undergoing ovarian stimulation, with hormone concentrations in serum and follicular fluid assessed at five time points during final follicle maturation; and (iii) 12 women undergoing fertility preservation, with hormone concentrations evaluated via the follicular fluid of small antral follicles.

RESULTS:

In the early follicular phase, theca cells primarily synthesized 17-OH-P4 while granulosa cells produced limited P4, maintaining the P417-OH-P4 ratio <1. As follicles reached follicle selection at a diameter of approximately 10 mm, P4 synthesis in granulosa cells was up-regulated, but P4 was mainly accumulated in follicular fluid. During final maturation, enhanced activity of the enzyme HSD3B2 in granulosa cells enhanced P4 production, with the P417-OH-P4 ratio increasing to >1. The concentration of 17-OH-P4 in the luteal phase was similar to that in the follicular phase, but P4 production increased in the luteal phase, yielding a P417-OH-P4 ratio significantly >1.

CONCLUSIONS:

The P417-OH-P4 ratio reflects the activity of granulosa cells and theca cells during the follicular phase and following luteinization in the corpus luteum. Managing the function of granulosa cells is key for reducing the concentration of P4 during ovarian stimulation, but the concerted action of FSH and LH on granulosa cells during the second half of the follicular phase makes this complex.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Progesterona / Células Tecais / Líquido Folicular / Células da Granulosa Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Progesterona / Células Tecais / Líquido Folicular / Células da Granulosa Idioma: En Ano de publicação: 2024 Tipo de documento: Article