Soluble CD14 produced by bovine mammary epithelial cells modulates their response to full length LPS.
Vet Res
; 55(1): 76, 2024 Jun 12.
Article
em En
| MEDLINE
| ID: mdl-38867337
ABSTRACT
Bovine mastitis remains a major disease in cattle world-wide. In the mammary gland, mammary epithelial cells (MEC) are sentinels equipped with receptors allowing them to detect and respond to the invasion by bacterial pathogens, in particular Escherichia coli. Lipopolysaccharide (LPS) is the major E. coli motif recognized by MEC through its interaction with the TLR4 receptor and the CD14 co-receptor. Previous studies have highlighted the role of soluble CD14 (sCD14) in the efficient recognition of LPS molecules possessing a full-length O-antigen (LPSS). We demonstrate here that MEC are able to secrete CD14 and are likely to contribute to the presence of sCD14 in milk. We then investigated how sCD14 modulates and is required for the response of MEC to LPSS. This study highlights the key role of sCD14 for the full activation of the Myd88-independent pathway by LPSS. We also identified several lncRNA that are activated in MEC in response to LPS, including one lncRNA showing homologies with the mir-99a-let-7c gene (MIR99AHG). Altogether, our results show that a full response to LPS by mammary epithelial cells requires sCD14 and provide detailed information on how milk sCD14 can contribute to an efficient recognition of LPS from coliform pathogens.
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Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
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Receptores de Lipopolissacarídeos
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Células Epiteliais
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Glândulas Mamárias Animais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article