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Prostate cancer reshapes the secreted and extracellular vesicle urinary proteomes.
Khoo, Amanda; Govindarajan, Meinusha; Qiu, Zhuyu; Liu, Lydia Y; Ignatchenko, Vladimir; Waas, Matthew; Macklin, Andrew; Keszei, Alexander; Neu, Sarah; Main, Brian P; Yang, Lifang; Lance, Raymond S; Downes, Michelle R; Semmes, O John; Vesprini, Danny; Liu, Stanley K; Nyalwidhe, Julius O; Boutros, Paul C; Kislinger, Thomas.
Afiliação
  • Khoo A; Department of Medical Biophysics, University of Toronto, Toronto, ON, M5G 1L7, Canada.
  • Govindarajan M; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Qiu Z; Department of Medical Biophysics, University of Toronto, Toronto, ON, M5G 1L7, Canada.
  • Liu LY; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Ignatchenko V; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90024, USA.
  • Waas M; Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Macklin A; Department of Urology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Keszei A; Institute for Precision Health, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Neu S; Department of Medical Biophysics, University of Toronto, Toronto, ON, M5G 1L7, Canada.
  • Main BP; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Yang L; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90024, USA.
  • Lance RS; Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Downes MR; Department of Urology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Semmes OJ; Institute for Precision Health, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Vesprini D; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Liu SK; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Nyalwidhe JO; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Boutros PC; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 2C1, Canada.
  • Kislinger T; Division of Surgery, Urology, Sunnybrook Health Sciences Centre, Toronto, ON, M4N 3M5, Canada.
Nat Commun ; 15(1): 5069, 2024 Jun 13.
Article em En | MEDLINE | ID: mdl-38871730
ABSTRACT
Urine is a complex biofluid that reflects both overall physiologic state and the state of the genitourinary tissues through which it passes. It contains both secreted proteins and proteins encapsulated in tissue-derived extracellular vesicles (EVs). To understand the population variability and clinical utility of urine, we quantified the secreted and EV proteomes from 190 men, including a subset with prostate cancer. We demonstrate that a simple protocol enriches prostatic proteins in urine. Secreted and EV proteins arise from different subcellular compartments. Urinary EVs are faithful surrogates of tissue proteomes, but secreted proteins in urine or cell line EVs are not. The urinary proteome is longitudinally stable over several years. It can accurately and non-invasively distinguish malignant from benign prostatic lesions and can risk-stratify prostate tumors. This resource quantifies the complexity of the urinary proteome and reveals the synergistic value of secreted and EV proteomes for translational and biomarker studies.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Proteoma / Vesículas Extracelulares Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Proteoma / Vesículas Extracelulares Idioma: En Ano de publicação: 2024 Tipo de documento: Article